2x PCR mix

2x PCR mix is optimized mixture. It contains Taq polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x PCR mix is designed to allow the user to quickly and easily prepare the mixture of reaction. The PCR mix may amplify products up to 3 kb and the products can be cloned into T-vector directly.
of nucleotides into duplex DNA in the 5’→3′ direction.
Pfu DNA polymerase possesses 3’→5′ exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high- fidelity. The fragments of Pfu DNA polymerase
generated are blunt-ended.