DNA Safe Stains
2X Pfu mix
Description
Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5′-3′ direction.
Pfu DNA polymerase possesses 3′-5′ exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of Pfu DNA polymerase
generated are blunt-ended.
2X Pfu Mix
containing 3.6mM MgCl2
Application
Amplification of DNA fragments is up to 7.5 kb Proof reading function.
Cat No | Pack size | Conc. |
TAMB09Z-500 | 3×1.7 mL | 2x |
TAMB09Z-2500 | 5x3x1.7 mL | 2x |
Storage conditions
long time at -20°C
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