DNA Safe Stains
2X RT Master Mix
Description
2X RT master mix is designed for convenient and efficient cDNA synthesis. The 2X pre-mixed reagent containing M-MLV RTase, Random 6mers, oligo dT ,dNTP mixture and reaction buffer. The reverse transcription reaction just add RNA and water. The protocol is easy and the reaction can be completed in short time.
Storage condition
long time at -20°C
2X RT Master Mix
Containing M-MLV reverse transcriptase, reaction
buffer, dNTP, oligo dT primers, random hexamers
and stabilizer.
Application
cDNA synthesis
PCR screening
Real-time PCR
| Cat No | Pack size | Pack size |
| TAMB18Z-50 | 50 ul | 2x |
| TAMB18Z-2500 | 2500 ul | 2x |
Activity Assay
200 units of enzyme are used to produce cDNA from 1µg of a 1.2kb control RNA. The minimum specification is 120ng of first strand cDNA made from 1µg of RNA. The cDNA product must be >90% full length.
Contaminant Activity
To test for endonuclease activity, 1µg of Type I supercoiled plasmid DNA is incubated with 500 units of M-MLV Reverse Transcriptase in 1X Reaction Buffer for one hour at 37°C.
Physical Purity
The purity is >90% as judged by SDS-polyacrylamide
gels with Coomassie® blue staining.
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