Description |
2X Redy Mix is optimized mixture. It contains Taq polymerase, reaction buffer, dNTP, enhancer and red dye as 2-fold concentration. 2x Redy mix is designed to allow the user to quickly and easily prepare the mixture of reaction and ready loading.
Storage condition |
long time at -20°C
Functional Assay |
It is tested for performance in the polymerase chain reaction (PCR) to amplify a 500 bp region of the actin cDNA gene. The resulting PCR product is visualized on an ethidium bromide-stained agarose gel.
Contaminant Assay |
No contaminating endonuclease or exonuclease activity detected.
Application |
- Amplification of DNA fragments up to 5 kb
- Labelling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
- Cycle sequencing
- Generation of PCR product for TA cloning
Cat No |
Pack size |
Conc. |
| TAMB06Z-500 | 3×1.7 mL | 2x |
| TAMB06Z-2500 | 5x3x1.7 mL | 2x |
PCR cycles program |
| Step | Temperature | Time | Cycle |
| Initial denaturation | 94-95ºC | 1-3 mins | 1 |
| Denaturation | 94-95ºC | 10-60sec | 25-35 |
| Annealing | 50-68ºC | 10-30sec | |
| Extension | 72ºC | 1min/1kb | |
| Final extension | 72ºC | 1-10 mins | 1 |
IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature.
Shipping and Storage conditions |
Shipping and temporary storage at -20 and for up to 1 month at room temperature has no detrimental effects on the quality of TnATaq DNA polymerase.
