2X Hi Fi mix

Hi Fi polymerase synthesizes higher yields of PCR product from genomic DNA, cDNA, bacterial cultures. It has 2.5 hours half life at 96°C and is easy to amplify PCR product of G-C rich or secondary  structure DNA.

2X Hi Fi Mix is optimized mixture. It contains Hi Fi polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x Hi Fi mix is designed to allow the user to quickly and easily prepare the  mixture of reaction. The 2x Hi Fi mix can amplify PCR products up to 10-15 kb and the products can be cloned into T-vector directly.

long time at -20°C

short time at  4 °C

containing 3.6mM MgCl₂

• Target of DNA fragments is up to 20 kb
• Proof reading function
• Amplification of genomic DNA