Description

HyFectTM DNA Transfection Reagent is a low cell toxicity and non-liposome reagent optimized for DNA delivery. It provides effectively, reproducibly, and affordable benefits for scientific research.

Protocol
  1. Cell preparation: Cells should be seeded before 16 to 20 hours prior to transfection with around 70% confluency The medium should be refreshed 30 mins before transfection Usually, culture media with serum does not affect transfection.
  2. DNA preparation: DNA plasmid for transfection should be with high purity (A260/A280 = 1.8-1.9) to ensure efficient transfection mixture preparation.
  3. Mixture preparation: Guideline of DNA plasmid and HyFectTM DNA Transfection Reagent amount and ratio refer to table 1. In brief, plasmid and transfection reagent are diluted in serum-free culture medium for 5 mins then mixed together and tip gently for another 25 mins.
  4. Transfection: Add mixtures into cell culture dish / plate The mixture could be removed after 6 to 48 hours and refilled with culture medium.

Cat No

Pack size
TAMB33L 500 uL

Storage condition

Store at -20 °C for long term

Table 1. Recommended formula of transfection mixture
Culture Dish/Plate Media Volume Plasmid/Serum-free medium HyFectTM DNA Transfection Reagent/Serum-free medium
96-well 100 μL 250 ng/ 10 μL 0.75 μL/ 10 μL
24-well 500 μL 500 ng/ 25 μL 1.5 μL/ 25 μL
12-well 700 μL 750 ng/ 35 μL 2.25 μL/ 35 μL
6-well 1 mL 1 μg/ 50 μL 3 μL/ 50 μL
6 cm 3 mL 2.5 μg/150 μL 7.5 μL/150 μL
10 cm 6 mL 5 μg/300 μL 15 μL/300 μL
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