Description |
Fluorescent dye (FAM) conjugated Annexin V is highly purified product. During early apoptosis, cells will translocate membrane phosphatidylserine (PS) from the inner face of the membrane to the cell surface. Propidium iodide (PI) is a common fluorescent dye to detect DNA. It can be used in flwcytometry to evaluate the cell cycle and cell viability during apoptosis. The product can be used in one-step staining procedure without wash step within 20 minutes.
Protocol |
- Collect 1-5×105 cells in the flow tube by centrifugation.
- Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
- Re-suspend cell in 500 μL of 1X Binding Buffer.
- Add 5 μL of Annexin V- FAM and 5 μL of PI, gently mix the cells and incubate for 15-20 min at RT in the dark.
- After incubation, the samples should be kept on ice andperform flow cytometry using filters appropriated for fluorescein (FITC, corresponding to Annexin V-FAM).
Storage condition |
Store at 4 °C in the dark. All reagents are stable for one year under proper storage conditions.
Package size and Components |
| Cat. 32111 | 25 rxn |
| Annexin V- FAM | 1 X 0.125 mL |
| PI | 1 X 0.125 mL |
| 10X Binding Buffer | 1 X 2 mL |
| Cat. 32112 | 50 rxn |
| Annexin V- FAM | 1 X 0.25 mL |
| PI | 1 X 0.25 mL |
| 10X Binding Buffer | 2 X 2 mL |
| Cat. 32113 | 100 rxn |
| Annexin V- FAM | 1 X 0.5 mL |
| PI | 1 X 0.5 mL |
| 10X Binding Buffer | 3 X 2 mL |
