BioSpot ISH Kit For DIG probe

  • BioSpot ISH Kit For DIG probe

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

Description

The BioSpot ISH Kit For DIG probe is designed to be used for the detection of digoxigenin (DIG) labeled probes in either formalin-fixed, paraffin-embedded tissue or cell samples by chromogenic in situ hybridization (CISH).
The kits have been developed using a hyperlabelling technology used to directly label probe with HRPs. The kit included complete pretreatment, labeling, blocking, washing and detection system. It also could be used for animal and plant samples in research filed.

TASH01D with DAB chromogen
TASH01A with AEC chromogen

TASH01D-datasheets
TASH01A-datasheets
Cat No Pack size Tests
TASH01D-50T 6mL 50T
TASH01D-100T 12mL 100T
TASH01A-50T 6mL 50T
TASH01A-100T 12mL 100T
Contents :
Hydrogen peroxidase block
High effect immunoblock
Rabbit probe HRP conjugated
DAB buffer
DAB chromogen (20X)

BioSpot ISH Kit For Biotin probe

  • BioSpot ISH Kit For Biotin probe

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

Description

The BioSpot ISH Kit For Biotin probe is designed to be used for the detection of Biotin labeled probes in either formalin-fixed, paraffin-embedded tissue or cell samples by chromogenic in situ hybridization (CISH).
The kits have been developed using a hyperlabelling technology used to directly label probe with HRPs. The kit included complete pretreatment, labeling, blocking, washing and detection system. It also could be used for animal and plant samples in research filed.

TASH02D with Biotin chromogen
TASH02A with Biotin chromogen

TASH02D-datasheets
TASH02A -datasheets
Cat No Pack size Tests
TASH02D-50T 6mL 50T
TASH02D-100T 12mL 100T
TASH02A-50T 6mL 50T
TASH02A-100T 12mL 100T
Contents :
Hydrogen peroxidase block
20X SSC buffer
DAB or AEC chromogen
Biotin Detection system

BioSpot ISH Kit with FAM For DIG Probe

  • BioSpot ISH Kit with FAM For DIG Probe

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

datasheets

Description

The Biospot kit F488 is designed to be used for the detection of digoxigenin (DIG)-labeled probes in either formalin-fixed, paraffin-embedded tissue or cell samples by fluorescence in situ hybridization (FISH).

Interpretation of results must be made within the context of the patient’s clinical history with respect to further clinical and pathologic data of the patient by a qualified pathologist.

Cat No Pack size
TASH01F-50 50 reactions
TASH01F-100 100 reactions
Contents :
Pretreatment kit
Heat Pre-treatment Solution,20x
Enzyme Solution
SSC buffer,20x
Hu DNA (+) control probe 5′-DIG
Ms DNA (+) control probe 5′-DIG
RNA (+) control probe 5′-DIG
Detection system
Hi-Effect Block reagent
Anti-DIG antibody
F488 labeling
DAPI Solution

BioTnA In Situ PCR kit with DAB Brown for DIG labeled probe

  • BioTnA In Situ PCR kit with DAB Brown
    for DIG labeled probe

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

datasheets

Description

The BioTnA In Situ PCR Kit with DAB is designed to be used for the detection of digoxigenin (DIG)-labeled probes hybridized specific DNA sequence through PCR application in either formalin-fixed, paraffin-embedded tissue or cell samples by chromogenic in situ hybridization (CISH).
Interpretation of results must be made within the context of the patient’s clinical history with respect to further clinical and pathologic data of the patient by a qualified pathologist.

Cat No Pack size
TAISP01D-50 50 reactions
TAISP01D-100 100 reactions
I. primer design
II. Sample preparing1.dewax
2.rehydration
III. pretreatment

1.De-peroxidase
2.Heat per-treatment
3.Lysis per-treatment
4.Enzyme pre-treatment
IV. PCR Step

seal the cover slip in sealing gel
V. Post-PCR Fixation
VI.In Situ Hybirdiaztion
VII.Detection

1.anti-Dig
2.HRP Labeling
3.DAB working solution
Contents :
Pre-PCR treatment
Heat Pre-treatment Solution,20x
Hydrogen Peroxidase Block
Lysis buffer
Enzyme Solution
PCR reagent
PCR reaction mixture
Control primer set
Post-PCR treatment
Fixation solution
SSC buffer,20x
Wash buffer,50X
Detection kit
Hybridization buffer
Sealing gel
Universal probe DIG labeled
Control probe DIG labeled
Hi-Effect Block reagent
Anti-DIG antibody
Polymer HRP labeling
DAB buffer
DAB chromogen,20x
Nuclear Blue Solution
Slide
Negative control slide*2
Control slide*4

BioTnA In Situ RT-PCR Kit with DAB Brown for DIG labeled probe

  • BioTnA In Situ RT-PCR Kit with
    DAB Brown for DIG labeled probe

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

datasheets

Description

The BioTnA In Situ RT-PCR Kit with DAB is designed to be used for the detection of
digoxigenin (DIG)-labeled probes hybridized specific RNA sequence through PCR
application in either formalin-fixed, paraffin-embedded tissue or cell samples by
chromogenic in situ hybridization (CISH).
Interpretation of results must be made within the context of the patient’s clinical history
with respect to further clinical and pathologic data of the patient by a qualified
pathologist.

Cat No Pack size
TAISP0D-50 50 reactions
TAISP0D-100 100 reactions
I. primer design
II. Sample preparing1.dewax
2.rehydration
III. pretreatment

1.De-peroxidase
2.Heat per-treatment
3.Lysis per-treatment
4.Enzyme pre-treatment
IV. PCR Step

seal the cover slip in sealing gel
V. Post-PCR Fixation
VI.In Situ Hybirdiaztion
VII.Detection

1.anti-Dig
2.HRP Labeling
3.DAB working solution
Contents :
Pre-PCR treatment
Heat Pre-treatment Solution,20x
Hydrogen Peroxidase Block
Lysis buffer
Enzyme Solution
PCR reagent
PCR reaction mixture
Control primer set
Post-PCR treatment
Fixation solution
SSC buffer,20x
Wash buffer,50X
Detection kit
Hybridization buffer
Sealing gel
Universal probe DIG labeled
Control probe DIG labeled
Hi-Effect Block reagent
Anti-DIG antibody
Polymer HRP labeling
DAB buffer
DAB chromogen,20x
Nuclear Blue Solution
Slide
Negative control slide*2
Control slide*4

TUNEL Apoptosis Assay Kit

  • TUNEL Apoptosis Assay Kit

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

datasheets

Description

The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, Digoxigenin-nucleotide is labeled at the DNA 3´-OH ends using the
natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, mouse anti- DIG and HRP labeled polymer bind to these Digoxigenin nucleotides,
which are detected using the peroxidase substrate, 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Cat No Pack size
TAAP01D-20 20 reactions
TAAP01D-50 50 reactions
TAAP01D -100 100 reactions
Contents :
Proteinase K
Permeabilization buffer
TdT Reaction Buffer
TdT Enzyme Reagent
TdT Label Reagent
Background reducing buffer
anti- DIG
HRP labeled polymer
DAB Chromogen (20x)
DAB Buffer

TUNEL Apoptosis Assay Kit(FAM)

  • TUNEL Apoptosis Assay Kit(FAM)

    Expired date: 18-24 months

    Storage condition :Store at 2-8 °C

datasheets

Description

The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, Digoxigenin-nucleotide is labeled at the DNA 3´-OH ends using the
natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The mouse anti- DIG bind to these Digoxigenin nucleotides, which are detected by following the anti-Mouse IgG (H+L)-FAM. The nuclei of apoptotic cells should be observed green color under fluorescence microscope.

Cat No Pack size
TAAP01F-20 20 reactions
TAAP01F-50 50 reactions
TAAP01F-100 100 reactions
Contents :
Proteinase K
Permeabilization buffer
TdT Reaction Buffer
TdT Enzyme Reagent
TdT Label Reagent
Background reducing buffer
anti- DIG
anti-mouse IgG (H+L)-FAM(488)
DAPI solution
Aqua mounting medium