TnATaq DNA polymerase

TnATaq  DNA  polymerase is a thermostable enzyme isolated from E. coli which encodes Taq DNA polymerase gene. This enzyme contains 5’-3’ poly merase and 5’-3’ exonuclease activity.

Storage buffer

50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA,

10X reaction buffer

Containing 15mM MgCl2

Unit description

One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl2, 200uM each of dATP, dCTP, dGTP, dTTP, 10 mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 ml.

Storage buffer

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at -20ºC, 100 mM KCl, 0.1 mM EDTA.

Source

E coli clone

Quality control

The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE – 95 kD band. Activity and stability tested via thermo-cycling. The error rate per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 10-4.
Estimated half life at 95ºC is 0.5 hours.