Hi Fidelity polymerase
Description
Hi Fidelity polymerase is a mixture of thermostable enzymes. It is specifically developed to synthesize length of PCR product up to 25 kb and with low error rate. Hi Fi polymerase synthesizes higher yields of product from genomic DNA, cDNA, and bacterial cultures. It has 2.5 hours half life at 96oC and easily amplify PCR product of G-C rich or secondary structure DNA by adding G- C rich buffer.
Storage condition
-20°C
10X reaction buffer
Buffer containing 25mM MgCl2
Unit description
One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl2, 200uM each of dATP, dCTP, dGTP, dTTP, 10 mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 ml.
Template
Hi Fidelity Polymerase is suitable for amplifying targets up to 25 kb from the following templates:
Genomic DNA: 10–200 ng
Plasmid DNA : 1–5 ng
cDNA : ~100 ng starting total RNA
Amplification of longer targets (up to 15 kb) may be possible, but may require more template and longer elongation times.
Cat No | Pack size | Conc. |
TAMB03Z-500 | 500U | 5U/uL |
TAMB03Z-2500 | 2500U | 5U/uL |
PCR cycles program | |||
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Step | Temperature | Time | Cycle |
Initial denaturation | 94-95°C | 1-3 mins | 1 |
Denaturation | 94-95°C | 10-60sec | 25-35 |
Annealing | 50-68°C | 10-30sec | |
Extension | 72°C | 1min/1kb | |
Final extension | 72°C | 1-10 mins | 1 |
IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature. |
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Shipping and Storage conditions
Shipping and temporary storage at -20 and for up to 1 month at room temperature has no detrimental effects on the quality of Hot start DNA polymerase.