2X Pfu mix

Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5′-3′ direction.
Pfu DNA polymerase possesses 3′-5′ exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of Pfu DNA polymerase
generated are blunt-ended.

2X Pfu Mix

containing 3.6mM MgCl2

Application

Amplification of DNA fragments is up to 7.5 kb Proof reading function.