HyFectTM DNA Transfection Reagent

HyFectTM DNA Transfection Reagent is a low cell toxicity and non-liposome reagent optimized for DNA delivery. It provides effectively, reproducibly, and affordable benefits for scientific research.

1. Cell preparation: Cells should be seeded
before 16 to 20 hours prior to transfection
with around 70% confluency The medium
should be refreshed 30 min before transfec
tion Usually, culture media with serum does
not affect transfection.

2. DNA preparation: DNA plasmid for transfec
tion should be with high purity (A260/A280 =
1.8-1.9) to ensure efficient transfection
mixture preparation.

3. Mixture preparation: Guideline of DNA
plasmid and HyFectTM DNA Transfection
Reagent amount and ratio refer to table 1. In
brief, plasmid and transfection reagent are
diluted in serum-free culture medium for 5
min then mixed together and tip gently for
another 25 min.

4. Transfection: Add mixtures into cell culture
dish / plate The mixture could be removed
after 6 to 48 hours and refilled with culture
medium.

Store at -20 °C for long term