DNA Safe Stains

DNA Safe Stains is a safe nucleic acid stain to replace traditional ethidium bromide (EtBr, a potent mutagen) for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gel. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. DNA Safe Stains products are non-carcinogenic by the Ames-test (negative results in both mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests).

1. Prepare hot melting agarose gel solution
(concentration from 0.5-2.5%).

2. Add 1 μL of DNA Safe Stains to every 20 mL
of gel solution. Swirl gently to mix the solu
tion and avoid forming bubbles.

3. Allow the agarose gel to cool until solidified.
Load samples on the gel and perform electro
phoresis.

4.  Visualize the bands under UV illumination.

Store at 4 °C