Author Archives: andrew

Pfu DNA polymerase

Description Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for…

Hi Fidelity polymerase

Description Hi Fidelity polymerase is a mixture of thermostable enzymes. It is specifically developed to synthesize length of PCR product up to 25 kb and with low error rate. Hi Fi polymerase synthesizes higher yields of product from genomic DNA, cDNA, and bacterial cultures. It has 2.5 hours half life at 96oC and easily amplify…

Hot start Taq DNA polymerase

Description Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts. Storage buffer 50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% glycerol. 10X reaction buffer Buffer…

TnATaq DNA polymerase

Description TnATaq  DNA  polymerase is a thermostable enzyme isolated from E. coli which encodes Taq DNA polymerase gene. This enzyme contains 5’-3’ poly merase and 5’-3’ exonuclease activity. Storage buffer 50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% glycerol. 10X reaction buffer Containing 15mM MgCl2 Unit description One unit is…