Hi Fidelity polymerase

Hi Fidelity polymerase is a mixture of thermostable enzymes. It is specifically developed to synthesize length of PCR product up to 25 kb and with low error rate. Hi Fi polymerase synthesizes higher yields of product from genomic DNA, cDNA, and bacterial cultures. It has 2.5 hours half life at 96oC and easily amplify PCR product of G-C rich or secondary structure DNA by adding G- C rich buffer.

Storage condition

-20°C

10X reaction buffer

Buffer containing 25mM MgCl2

Unit description

One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl2, 200uM each of dATP, dCTP, dGTP, dTTP, 10 mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 ml.

Template

Hi Fidelity Polymerase is suitable for amplifying targets up to 25 kb from the following templates:
Genomic DNA: 10–200 ng
Plasmid DNA : 1–5 ng
cDNA : ~100 ng starting total RNA
Amplification of longer targets (up to 15 kb) may be possible, but may require more template and longer elongation times.