2X Pfu mix

Pfu DNA polymerase is a  thermostable enzyme  isolated from Pyrococcus furiousus. The enzyme  replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5′-3′ direction.

Pfu DNA polymerase possesses 3′-5′ exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of  Pfu DNA polymerase generated are blunt-ended.

2X Pfu mix is optimized mixture. It contains Pfu  polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x Pfu mix is designed to allow the user for quickly and easily preparing reaction mixture. The 2x Pfu mix can amplify PCR products up to 3-5 kb.

long time at -20°C

containing 50mM MgCl₂

• Amplification of DNA fragments is up to 7.5 kb
• Proof reading function.