Description

2x qPCR Master mix is designed for quantitative real-time analysis of DNA samples.

  • Strong signals and high sensitivity due to fluorescent dye.
  • High specificity – no primer dimers, no NTC signal.
  • Optimized 2x qPCR Master mixes for different real-time PCR instruments. Master mix formulations are optimized for different machines.

2x qPCR Master mix is ideally suited for:

  • Gene expression analysis
  • Microarray validation
  • Viral load determination qRT-PCR

Components

2x qPCR Master mix is a 2x mixture of dNTPs, Hotstart Taq  polymerase, MgCl2, fluorescent detection dye, reference dye (optional), and buffer components.

Storage condition

long time at -20°C

Cat No

Pack size

Description

qPCR Instruments

TAMB11Z-500 3x 1.68 mL
5x3x1.68 mL
2X qPCR master mix with ROX ABI,7000,7300,7700,7900, stepOne Plus StepOne™ Eppendorf Realplex 4 ABI7500 Stratagene Mx3000,Mx3005, Mx4000
TAMB11Z-2500 3x 1.68 mL
5x3x1.68 mL
2x qPCR master mix BioRad CFX96 Roche LightCycler 480 MJ Research Opticon and Opticon 2 MJ Research Chromo 4 Corbett Rotor-gene 600,3000 Eppendorf Realplex 2Product Application
2X qPCR Master mix figure1
Comparison: BioTnA and another
2X qPCR Master mix figure2
The real time PCR results of different concentration of β-actin cDNA
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