2X RT Master Mix

2X RT master mix is designed for convenient and efficient cDNA synthesis. The 2X pre-mixed reagent containing M-MLV RTase, Random 6mers, oligo dT, dNTP mixture and reaction buffer. The reverse transcription reaction just add RNA and water. The protocol is easy and the reaction can be completed in short time.

long time at -20°C

Containing M-MLV reverse transcriptase, reaction buffer, dNTP, oligo dT primers,  random hexamers  and stabilizer.

• cDNA synthesis
• PCR screening
• Real-time PCR

200 units of enzyme are used to produce cDNA from 1µg of a 1.2kb control RNA. The minimum specification is 120ng of first strand cDNA made from 1µg of RNA. The cDNA product must be >90% full length.

To test for endonuclease activity, 1µg of Type I supercoiled plasmid DNA is incubated with 500 units of M-MLV Reverse Transcriptase in 1X Reaction Buffer for one hour at 37°C. Following incubation, the supercoiled DNA is visualized on an ethidium bromide-stained agarose gel to verify the absence of visible nicking or cutting (analysis on 0.4µg of DNA).

The purity is >90% as judged by SDS-polyacrylamide gels with Coomassie® blue staining.