Hot start Taq DNA polymerase

Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts.

50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA,

1mM DTT, 0.5mM PMSF , 50% glycerol.

Buffer containing 25mM MgCl₂

One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl₂, 200uM each of dATP, dCTP, dGTP, dTTP, 10 mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 μl.

E coli clone

• Hot Start and real time PCR
• Multiplex PCR
• Amplification of complex genomic and cDNA

IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature.

Shipping and temporary storage at -20 and for up to 1 month at room temperature has no detrimental effects on the quality of Hot start Taq DNA polymerase.