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5X GC rich buffer

Description Improve the amplification of GC-rich targets from genomic DNA or cDNA template.  The GC rich buffer can reducing  the denature temperature of template. Storage condition long time at -20°C Application GC-rich targets Repetitive sequences Templates with varying  GC content Improves fidelity of PCR amplification Multiplex PCR Denaturation The first denaturation step must be more than…

2X Pfu mix

Description Pfu DNA polymerase is a  thermostable enzyme  isolated from Pyrococcus furiousus. The enzyme  replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'-3' direction. Pfu DNA polymerase possesses 3'-5' exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended…

2X Hot start mix

Description Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot-start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts. 2X Hot Start mix is optimized mixture. It contains Hot Start Taq polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration.…

2X Hi Fi mix

Description Hi Fi polymerase synthesizes higher yields of PCR product from genomic DNA, cDNA, bacterial cultures. It has 2.5 hours half life at 96°C and is easy to amplify PCR product of G-C rich or secondary  structure DNA. 2X Hi Fi Mix is optimized mixture. It contains Hi Fi polymerase, reaction buffer, dNTP and enhancer as…