Pfu DNA polymerase

Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5’→3′ direction.

Pfu DNA polymerase possesses 3’→5′ exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high- fidelity. The fragments of Pfu DNA polymerase generated are blunt-ended.

2 x10-6

One unit of Pfu DNA Polymerase incorporates 10 nmol of dNTP into acid-insoluble material in 30 mins at 74°C.

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at -20ºC, 100 mM KCl, 0.1 mM EDTA.

E coli clone

The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE – 95 kD band. Activity and stability tested via thermo-cycling.

IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature.

Shipping and temporary storage at -20 and for up to 1 month at room temperature has no detrimental effects on the quality of  Pfu DNA polymerase.