DNA Safe Stains
T4 DNA ligase
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′-phosphate and 3‘-hydroxyl ends in double strand DNA or RNA with blunt or cohesive-end termini
Storage condition
long time at -20°C
10X Ligation Buffer
Contain with Tris-HCl, MgCl2, DTT, ATP
Storage buffer
20 mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA and 50% glycerol
Inactivation
By heating at 65°C for 10min
Unit definition
0.01 Weiss unit is defined as the amount of enzyme required to catalyze the ligation of greater than 95% of the Hind III fragments of 1mg of Lambda DNA at 16°C in 20 minutes
Applications
Joining double-stranded DNA with cohesive or blunt
termini and 3’T-A overhangs.
| Cat No | Pack size | Conc. |
| TAMB25Z-50 | 500 U | 5 U/uL |
| TAMB25Z-250 | 2500 U | 5 U/uL |
Additional information
If the reaction is blunt end or T-A ligation must have more units of T4 DNA ligase. T4 DNA ligase is strongly inhibited by NaCl or KCl if the concentration exceeds 200mM.
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